KASPar SNP Genotyping System
The KBiosciences PCR SNP genotyping system
is a novel homogeneous fluorescent genotyping system. We utilise a unique
form of allele specific PCR that is distinct and different to conventional
ARMS (Patent pending). It has been developed at KBiosciences and is currently
in use on a daily basis. It offers the simplest and most cost effective
way to determine SNP genotypes in the laboratory.
Recent News
Recently we had cause to compare and contrast KASP to Taqman in a relatively large study. The results were highly pleasing (for us!) and can be found by clicking here
Features
- Very high SNP to assay conversion rate (>90% in comparison to ~60% with Taqman)
- Flexible - Ability to perform direct or indirect assays. Works well in 96, 384 or even 1536-well plate formats
- Assays easily designed and optimized by end-users using Primer Picker free software
- Compatible with many detection instrument platforms from conventional plate readers to ABI Prism instrumentation
- Single-step, closed tube reactions
- The most cost-effective SNP typing system (up to 36x cheaper than Taqman)
- Universal SNP detection reagents
- No Need for costly, allele-specific fluorescent dual labelled oligonucleotides
- Genotype difficult SNPs without sequencing
- Accurate
- Reproducible
- Requires small amounts of sample material
- Closed-tube format minimises handling and risk of contamination
Kit Components
- Pack leaflet
- KASPar reagent
- Buffer optimisation reagents for AT and GC rich sequences
- Access code to free Primer Picker download
- KTaq DNA polymerase specific for KASPar
Prices:
Note: The price per reaction is based on a 10ul reaction performed in
a 96-well plate. It is perfectly possible and expected that reactions performed
in a 384-well plate will be carried out in a 5ul reaction thus halving the cost
/ reaction.
Methodology
The KASPar assay system relies on the discrimination power of a novel form
of competitive allele specific PCR to determine the alleles at a specific locus
within genomic DNA for SNP typing.
Traditionally, allele specific PCR (ARMs) has been shown to work by a number of groups worldwide. A number of improvements to this technique have been made in the past few years. The most significant of these is the use of 3' - 5' exonuclease deleted Taq DNA polymerases. These deleted Taq's increase the discriminating power of the technique, however the technique can still suffer from extension of the incorrect allele, providing false positive signals. KBiosciences has perfected this technique by employing a novel form of allele specific PCR that is distinct and different from ARMS. This increases the robustness and discriminating power of the technique and is currently in use on a daily basis at our facility
KBiosciences has spent a number of years perfecting this technique and is now pleased to release its novel Fluoresence resonance energy transfer (FRET) homogeneous format for this technique.
Validation
To ascertain the accuracy and reproducibility of the KASPar system a number
of studies have been performed to measure the level of concordance with Taqman
assays. To date we have carried out in excess of 10,000 genotypic comparisons.
The concordance rate has been shown to >99.5% with Taqman. We have also carried
out a number of blinded duplicate studies to determine the reproducibility of
the KASPar assay system. Without exception in over 20 of these studies involving
millions of genotypes we have shown the error rate and reproducibility to be
<0.3%.
Data
We have performed literally tens of millions of genotypes to date with the KASPar system. A selection of these are shown below:
More data can be found by clicking here
