KBioscience is pleased to offer its full length Hotstart Taq called KlearTaq
As with all our products Klear Taq is in use daily in our service laboratory. It has to be of both the highest quality and the most robust performance to meet our daily needs. It is supplied with a highly optimised reaction buffer which we have refined over 4 years of usage.
click here for 100u free sample of our KLAR taq
Purification
Klear Taq is produced by over-expression of the Taq DNA polymerase gene cloned into an E.coli vector. The production is a strictly controlled process in every way from bacterial growth times to batch size to intense QC procedures.
The enzyme is highly purified by a combination of differential thermal denaturation, size exclusion and ion exchange chromatography. Post purification, the enzyme is inactivated by a novel method (patent in preparation), resulting in an enzyme that gives highly specific and robust performance in PCR.
Quality Control
Each batch is quality controlled for both performance and quantity of active enzyme.
To assess performance Klear Taq is tested in a difficult PCR known to perform badly when non-Hot Start Taq polymerase is used (this PCR suffers badly from primer-dimer without the use of Hot Start Taq). The quality control procedure tests the inactivated (Hot Start) Klear Taq against the non-inactivated to confirm the success of the inactivation process.
To assess quantity each batch of Klear Taq is tested in an enzyme dilution series in comparison to previous batches of Klear Taq and to other manufacturers Taq. This QC procedure omits the more typical incorporation of radiolabelled thymidine into activated DNA. We have taken this atypical approach as the conventional assay has been shown in our hands to be unreliable and uninformative.
Uses
KlearTaq Hot Start DNA polymerase is a highly specific robust enyme that can be used for many PCR applications. It is suitable for all PCR applications and is a direct replacement for all leading commercial Hot Start Taq polymerases.
Validation, Testing & Performance
Klear Taq has been tested against all leading competitor enzymes and compares very favourably with all. We have used it in house for some time for the following applications, general PCR, library PCR, nested PCR & Whole genome amplification (PEP)
If you can find hotstart Taq cheaper please call us and we will guarantee to beat your current price !
Please enquire for a free 100U trial sample at info@kbioscience.co.uk