SNPline - the flexible genotyping solution
From 20 to more than 500,000 genotypes each day, SNPline provides
the ultimate solution for generation of flexible, high quality genotyping data.
SNPline modular laboratory instrumentation - the flexible genotyping solution
Adaptable to any throughput requirement
The SNPline system, produced by the laboratory instrument team at KBioscience, provides the ultimate solution
in flexible genotyping in any laboratory setting. Each SNPline can be provided with our proprietary Kraken
software package to enable complete experimental workflow management including assay design, extraction,
sequencing, genotyping and data analysis.
SNPline is available in SNPline Lite or SNPline XL versions and can be further customised to suit the needs of
any genotyping capacity and can be designed to incorporate existing laboratory equipment. From 20 to more
than half a million individual data points per day, the unique SNPline approach enables generation and
interpretation of data sets of any size to genotype SNPs, insertions or deletions.
SNPline modular workstation
Unrivalled flexibility
SNPline is available in multiple configurations and can be used in combination with your existing laboratory equipment,
enabling analysis of any number of samples for one or thousands of different SNPs.
• Run any number of samples and any number of assays
with no compromise on speed or increase in cost per analysis
• Flexibility to repeat only the downstream assays requiring further analysis
with no restriction on configuration as with array and chip based solutions
• Suitability for use with a variety of chemistry options including KASP,
TaqMan® or Invader®.
• Solutions for DNA prep and PCR in any plate density, including 1536.
Cost effectiveness
Significantly lower reagent costs and the ability to run assays in only those wells where data needs to be interpreted,
provides significant savings compared to array based systems where the need to repeat a particular assay can
necessitate a repeat of the entire data set. We use SNPline in our service laboratories and the development
of cost efficient, reliable systems has been integral to our success.
• Genotyping from as little as a penny or just a few cents per data point
• Requires minimal DNA and has no requirement for expensive sample clean-up or pre-amp preparation
prior to analysis
• Assay robots dispense reagents only into those MTP wells to be analysed
• Automated pipetting and reagent handling
• Experimental set-up and data analysis costs are significantly reduced with the SNPline in combination with on-board
Kraken workflow analysis software.
With expert product and technical support
Our scientists have more than 10 years genotyping experience using the SNPline systems in our service laboratories
in Europe and the USA. Our laboratory based, scientific support team are on hand to provide full product and technical
support where and when you need it. We can provide support for assays targeting difficult SNPs or InDels and have
extensive experience working with genomes from difficult organisms.
Kraken experimental design & data management software
Our proprietary Kraken software is a dedicated information and laboratory workflow manager
for all genotyping, sequencing, and extraction work, from sample storage to data analysis.
It has been developed by scientists for scientists for small and large projects.
We also offer a range of other genotyping software solutions including KlusterKaller
and SNPviewer.
KASP assays are available in two formats
to optimise flexibility and cost efficiency
KASP By Design
The KASP By Design - KBD - assay is our most cost effective solution for primer design and
assay development. The KBD service makes use of our Kraken assay development software,
to provide the best in-silico predicted assay, although the service does not include full assay
validation prior to shipment. A KBD assay is shipped in a 2D barcoded tube and comprises
the three Kraken designed oligos premixed at the appropriate concentration. When combined
with our KASPar mastermix your SNP genotyping will be ready to run. As with all our products
and services our technical support team is available to assist you with data interpretation and
trouble shooting if required.
KASP On Demand
The KASP On Demand - KOD - service includes full validation and assay optimisation
by our in-house genotyping experts prior to shipment of the assay. Using customer sequence
data, we optimise and fully validate the assay prior to shipping the oligonucleotides, which are
combined together in 2D barcoded tubes complete with full experimental protocols.
As with the KBD service, combination of the assay with our KASPar mastermix provides
the complete solution, ready to use.
SNPline and KASP genotyping
reagents - cost effective genotyping
Our KASP genotyping reagents for the detection of SNPs and InDels provide
a homogeneous, fluorescence based genotyping assay, utilising a unique form
of competitive, allele specific PCR that delivers extremely high levels of assay
robustness and accuracy. The KASP chemistry combines the use of a highly
specific 5' – 3' exonuclease deleted Taq DNA polymerase with two competitive,
allele specific, tailed forward primers and one common reverse primer.
The technology has been ptimised over many years in our own genotyping service
laboratories to provide unrivalled levels of SNP discrimination at a specific locus
on a genomic DNA sample. The KASP reagent system combined with our SNPline
instrumentation has underpinned the development of our genotyping service
laboratories and has enabled us to generate billions of genotypes for customers
worldwide.
• More than 500,000 genotypic comparisons showing a concordance rate >99.5%
with leading competitor technologies
• Over 20 independent blind duplicate studies involving more than 20 million data
points to validate reproducibility.
The cost effective, novel KASP chemistry provides
• Compatibility with 96, 384 and 1536 plate formats from 0.5 μL to 20 μL reaction
volumes
• 99.8%+ accuracy, independently assessed (reports are available on request)
• The ability to genotype difficult SNPs without sequencing - very high SNP to
assay conversion rate (>90%)
• Reduced starting material volumes with as little as 3ng DNA of starting mater utilised in the assay (dependent on genome size)
• Significantly reduced assay setup cost in comparison to other fluor labelled
techniques - no dual labelled probes required
• Utilises passive reference dye (ROX) which operates as an internal control
standard
• Compatibility with a wide range of fluorescence analysers
• Single-step, closed-vessel reaction which eliminates sample cross contamination.
Complementary products and services
• SNP assays (KASP On Demand & KASP By Design)
• Sanger sequencing
• Next generation sequencing services (Roche 454, Illumina HiSeq)
• DNA extraction services
• DNA extraction products (sbeadex®, KlearGene and mag™ kits)
• RNA extraction services
• Enzymes and PCR mix (KASPar, KlearKall, KlearTaq, KlearTaq Hifi)
• Whole Genome Amplification (WGA) kits and services
• DNA shearing instruments (Covaris)
• MTP heat sealers (Kube, FlexiSeal)
• MTP laser sealer (Fusion)
• Thermal cycling instruments (Hydrocycler)
• Assay dispensing systems (Meridian)
• MTP & seals for heat & laser (96, 384 & 1536)
• Software (SNPviewer, KlusterKaller, Kraken)
• DNA extraction instruments (oKtopure)
• DNA MTP stamping robot (repliKator)
Products
