Plasma extraction
Plasma: standard procedure calls for extraction with acetonitrile (3 times 3:1 acetonitrile:sample). The acetonitrile crashes out plasma proteins after the first addition, giving a very gummy protein pellet that is difficult to break-up for further extractions.
Current protocol 3:1 acetonitrile. vortex spatula.
Buy using the Covaris acoustics it is possible to greatly improve upon traditional methods.
By reducing ratio of Acetonitrile/Plasma to 1:1 then acoustically mixing, creates a very homogenous flocculent prep. Then add 2 parts acetonitrile for final extraction.
It is also possible to spin down pellet and cryo-prep to rapidly shatter the pellet then carry out extraction on increased surface area.
By carrying out the extraction in two parts there is NO gummy difficult to handle material. The final constituency is is very flocculent with a large surface area allowing highly efficient repeatable extraction
Image 1&2 1:1 acetonitrile initial acoustic mix followed by 3:1 acetonitrile extraction. Very flocculent end, large surface area for extraction.
Imge 3 Cryo-preped standard prep spun down pellet, fractured material then acoustic mix. settled
Unique Features & Benefits
| Feature | Benefit |
| Non-contact | Sample is not contaminated |
| Controllable mechanical energy | Dissolution is controllable for all application areas Increased biological activity in preparations |
| Electronic driven | Highly repeatable |
| Isothermal | No heat generated. |
| Fast | Many samples can be processed in very short time frames |
| Samples are closed tube | Safe – no aerosol risk |
| Scalable | Volumes from 200µl - 10mls |
| Automation enabled | E-200 can process microplates or racks of tubes |