Plate mixing 96-1536
The ability to ensure soluble compounds are in solution in HTS assays has not been possible… until now. The L-series instruments are designed for high-throughput fluidization, mixing, solvation, and dissolution in microplate formats.
The instruments are powered by the Covaris Adaptive Focused Acoustic (AFA) technology. AFA, a proprietary computer controlled, focused acoustic process based on shock wave physics, delivers controlled, precise, and accurate energy to a fluidic sample for unsurpassed mixing.
The benefits of AFA-enabled processes are now scaled to the highthroughput and high performance demands of HTS.
• non-contact, isothermal processing
• rapid transfer of non-ionizing energy
• reduced false negatives with uniform solvation
• improved assay performance; particularly in High Density Plates
Study
data shows an overallimprovement in assay performance in terms of Z’. This is particularly apparent in the case of the cAMP HTRF assay, where Z’ values are improved by a factor of 0.2.
Aditional benefits, particularly in the HTS scenario, are accelerated assay time frames, non-contact mixing, and ease of automation.
The time it takes for the assay to reach equilibrium is also reduced by 30% (and hence maximal signal is attained earlier). The reduction in both mixing time and time to reach maximal signal could increase plate throughput.
Application note -
Benefits of Covaris AFA Ultra Sonication on the HTS Process from Compound Master to HTS Assay Plate
Data generated also indicated the positive effect of acoustic mixing on compound solubility. SPA bead based assays should also benefit due to the increased collision frequency of the binding partners at the receptor-liquid-bead interface. Current assay performance can be compromised due to bead settling and inefficient static diffusion; resulting in long incubation times.
Video Controlled acoustic mixing 1536 plate
