Protocols - Application Note DNA shearing
Next Generation Sequencers benefit from a Next Gen DNA Shearing process (Overview)
Next Generation DNA Sequencers require stringent control of sample preparation protocols. As the cost and time involved in preparing samples for Next Gen systems are significant, every step (including DNA shearing) needs to be optimized to insure efficient amplification and accurate sequencing. For example, heat and/or enzymes may result in fragment bias. Both the precision and the accuracy of DNA sequencing benefit from unbiased fragment formation. The Covaris systems were designed to enable the highest quality, smallest base pair size sample preparation.
DNA Shearing
Protocol DNA shearing <1.5kb DNA Shearing with microTUBE
DNA shearing protocol for fragments 2.0kb (CLEAR tube)
DNA shearing protocol for fragments 3.0kb (BLUE tube)
DNA shearing protocol for fragments 5.0kb (RED tube)
SureSelect Target
Enrichment System Illumina Single-End
Sequencing Platform Library
Prep
Chromatin Shearing
Protocol Chromatin shearing without SDS detergent buffers
Protocol Chromatin shearing with SDS detergent buffers
Poster - Chromatin Shearing Based on Covaris AFA
