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Instrumentation

Covaris AFA
Flexi-Seal plate sealer
Fusion Laser sealer
Excision Laser piercer
Thermal cyclers
Scorpion Plate stacker
Colony Picker / Gridder

New FlexiSeal
plate sealer
flexiseal

Thermal Cyclers

KBioscience introduces a state of the art water bath HT-PCR system, the Hydrocycler, based on the proven original water-bath technology.

  1. Increased throughput
  2. Faster cycle time
  3. Higher quality results
  4. Improved reliability
  5. Multiple plate format no changing blocks
  6. Smaller footprint
  7. 16 & 32 plate system

Manual Hydrocycler 16 & 32manualvideo hydrocyclervideo New hydrocycler 4


Running up to 16x 384 well plates simultaneously, the Hydrocycler increases throughput and reproducibility at decreased cost, run time and space. Using the water-bath allows users to use 96, 384 and even 1536 plates in the same instrument.

 

16 plate hydrocycler
bench standing
32 plate hydrocycler
floor standing
thermal cycler hydro 32



The Technology
Hydrocycler thermal cyclers uses three computer PID controlled water baths to modulate the temperatures of the PCR reaction. A robotic arm is then used to move these plates between the three water baths, achieving a rapid thermal cycling profile. The result is higher throughput and higher data quality compared to standard thermal cyclers.

gui

Increasing your PCR throughput

The Hydrocycler is equipped with an easy quick-lock and release plate module for exchanging plates between runs. This HC-16 can hold up to 11 x 96 well plates or 16 x 384 well plates (depending on the plate type). The HC-32 can hold up to 22 x 96 well plates or 32 x 384 well plates (depending on plate types)

basketImproving reliability and reproducibility

Using a large mass of water as a source for conducting the energy transfer to your plates ensures both rapid thermal transfer and the best possible well-to-well / plate-to-plate uniformity.

In High Throughput PCR the elimination of these peltier based systems issue like edge effects and block-to-block reproducibility can be vital to the consistency of you products quality.

Saving your lab space

Designed to fit on the lab bench, the Hydrocycler takes merely the same space as 4 ordinary peltier PCR machines. The user can easily access the plate module and operate the touchpad control system whilst standing in front of the system.

IBesides holding many plates, the Hydrocycler (16 or 32) also increases throughput by faster cycle times.

Where cooling and heating speeds rely on peltier ramping temperatures in regular PCR machines, the Hydrocycler only needs ~ three seconds to change to the next water-bath. This relates in an overall reduction in PCR run time of up to 30%.

Text Box: Hydrocycler  15 min @ 95°C  35 Cycles of:  1min @ 95°C  1 min @ 58°C  1 min @ 72°C  Total: 1h 55min    Text Box: Peltier PCR  15 min @ 95°C  35 Cycles of:  1min @ 95°C  1 min @ 58°C  1 min @ 72°C  Total: 2h 45min

 

 

 

 

 

 

pcr gel

 

 

 

 


guiEasy programming

The touch screen user interface allows users to program, view and control all aspects of the Hydrocycler. The graphical user interface is easy to use and programmes can be stored on the Hydrocycler or a USB stick.

The GUI shows water-bath status, program progression and time to completion.

Saving cost of running and maintaining your PCR capacity
Constant cooling and heating of peltier PCR machines not only generates a lot of heat, but also consumes a lot of power. Keeping thoroughly insulated water-baths in the Hydrocycler at a steady temperature significantly decreases overall power consumption. The difference in power consumption between a Hydrocycler and the equivalent peltier PCR capacity on a yearly bases can add up to the same as the consumption of an average household !.

footprintInstrument Specifications:
• User-friendly graphical user interface
• Touch screen programmable PC
• 220v
• 784 mm (diameter) x 900mm (height)
• Weld seal basket for permanent sealing
• Compression basket for re-access to plates