KBioscience contacts

Main office
Tel +44 (0) 1992 470 757
info@kbioscience.co.uk


US Sales
Tel +1 978 232 9430
info@kbioscience.com

Site search

 

Genotyping submission steps 1-7

News: latest Nature paper
click here to download


NEW Zebra fish panel
zebrafish

Conferences:
PAG 2009 San Diego

Genotyping pipeline snp line

Kbioscience on the BBC
bbc





Genotyping Project Cost Calculator

Genotyping cost calculator




project with new SNPs using KASPar technology 1-3 weeks as above.
project.

Enter number of SNPs:
Enter number of Samples: (Minimum 22)

Total number of genotype tests

Setup unit price Setup cost
Genotyping unit price  Genotyping price
    Oligo restock
     
    Total cost

Project examples

 



Small scale study of 15 SNPs genotyped over 96 samples where no Assay on Demand (an alternative type of assay from ABI) SNP exists

  KBiosciences cost ABI Taqman Assay by Design
SNP assay design costs (validation) £1,200 £6,750
Genotyping cost £172.80 £388.80
Total £1,372.80 £7,138.80

NB. The ABI costs are based on list price quotations from a number of our customers and a 10ul reaction being used. (project cost calculator link)
Less then 1/4 the price

For any project whatever the size KBiosciences supplies the following items fully in the price

  • Labour
  • All plasticware (plates, tips, robot tips, seals)
  • Production of daughter plates from the master plates supplied to us
  • Data Analysis
  • All reagents inclusive of PCR enzymes etc..




Medium scale study of 100 SNPs typed over 2500 samples

  KBiosciences cost ABI Taqman Assay on Demand
SNP assay design costs (validation) £7,000 £21,000
Genotyping cost £17,500 £32,500
Total £24,500 £53,500

NB. The ABI costs are based on list price quotations from a number of our customers and a 5ul reaction being used with two aliquots of AOD demand probes being required
Half the price

For any project whatever the size KBiosciences supplies the following items fully in the price

  • Labour
  • All plasticware (plates, tips, robot tips, seals)
  • Production of daughter plates from the master plates supplied to us
  • Data Analysis
  • All reagents inclusive of PCR enzymes etc..




large study of 2000 SNPs over 1000 samples

If one is to contemplate purchasing the capital equipment to carry out the genotyping in-house then it is worth considering how much genotpying this equates to using our service. For example a capital equipment purchase of £250,000 would allow for a project of 2000 SNPs over 1000 DNA samples to be conducted. If the overheads of, consumables and staff are also calculated then this figure can be dramatically affected

  KBiosciences cost
SNP assay design costs (validation) £100,000
Genotyping cost £120,000
Total £240,000

For any project whatever the size KBiosciences supplies the following items fully in the price

  • Labour
  • All plasticware (plates, tips, robot tips, seals)
  • Production of daughter plates from the master plates supplied to us
  • Data Analysis
  • All reagents inclusive of PCR enzymes etc..




Speed congenics
example

For breeding / mapping a genetic trait in a mouse the use of genotyping can be employed.  It is imperative that the genotyping is carried out rapidly due to the lifecycle of the mouse.   For a generation of 22 mice using the KBioscience ready Mouse SNP panel to type 150 markers across a locus the following cost structure would be applicable.

Over 22 mice $ £
Mouse tail DNA extraction 170 88
Genotyping cost 1750 900
Fast turnaround charge (guaranteed 5 day turnaround) 850 450
Total 2,900 1,438

please contact us for detailed quote

NB. If you were to commit to a regular speed congenics order we would consider dropping the fast turnaround charge