kodKASPar On Demand for Fluidigm (KODF)
Assay design, manufacture testing and optimisation service

KBiosciences has carried out literally 100,000's of successful assay designs to date. We consider ourselves expert at getting even some of the most difficult assays to work

The KODF service entails the following

  1. E-mail us your sequence(s) of interest
  2. The design of the assay and the ordering of the Oligos is carried out in the KBioscience LIMS
  3. The oligonucleotides arrive & are combined to form an assay using 2D barcoded tubes
  4. The assay is validated over 44 random Caucasian DNA samples (or samples of your choice)
  5. If the assay requires optimisation we then carry this out in-house
  6. The assay is shipped to the customer along with the 2D barcode, name and any special conditions required for optimal performance

Performance
Typically we are able to achieve a success rate of >90% conversion to working assays. For more information see our recent in depth study in comparison to Taqman (click here)

Please note that KBioscience does not have a Fluidigm system, hence all assay validation will be conducted in 384 well plates

Delivery
The working aliquots will provide sufficient assay mix to genotype a guaranteed minimum of >2500 reactions on the Fluidigm platform

Assay Submission
To submit assays to our KODF service please fill in our customer application form (click here) (if applicable). Sequences along with customer details should then be e-mailed using the SNP submission form (click here) to kbd@kbioscience.co.uk

Delivery Times
We offer two speeds of delivery, normal and fast. The costs differ, due to the differing cost in oligo manufacture and prioritisation at KBioscience.
- Normal turnaround times are an estimated delivery of 2-4 weeks
- Fast turnaround times are a guaranteed delivery of under two weeks.

 KASP-On-Demand for Fluidigm– Validated SNP assay  - STANDARD turnaround
 Number of KODF assays    Product Code    Cost/assay STANDARD turnaround
 1-5    KBS-1005-001    $172    £100  
 6-10    KBS-1005-002    $146  £85  
 11-20    KBS-1005-003    $137    £80  
 21-100    KBS-1005-004    $129   £75  
 101-200    KBS-1005-005    $120    £70  
 200+    KBS-1005-006  enquire    enquire  
 KASP-On-Demand for Fluidigm– Validated SNP assay  - FAST turnaround
 Number of KODF assays    Product Code    Cost/assay FAST turnaround
 1-5    KBS-1009-001    $258    £130  
 6-10    KBS-1009-002    $232   £115  
 11-20    KBS-1009-003    $206    £110  
 21-100    KBS-1009-004    $189    £150  
 101-200    KBS-1009-005    $180   £100  
 200+    KBS-1009-006    enquire    enquire  
 KASP By Design – Non Validated SNP assay  
 Number of KBDF assays    Product Code      
 1-24   KBS -1013-001  $80   £50
 25+   KBS -1013-002  $50   £30